MechanoPro-DB

MechanoProtein DataBase (MechanoPro-DB)


Proteins from Force Spectroscopy Experiments and Molecular Dynamics Simulations

Name (Click for details) PDB ID SCOP annotation Highest unfolding forces/ Clamp forces [pN] Standard Deviation of force [pN] Velocity [nm/s] Loading Rate [pN/s] Xu [nm] ΔG [kBT] Contour Length [nm] Koff [s-¹] Temperature (°C ) Experimental Conditions Total Length (AA) Domain Coords 3D Structure (click on the protein) Organism Mutations Classification Technique Pulling Mode DOI Structure Released Authors Pulling Reference Unfolding Annotation Unfolding Pathway Mechanical Clamp Unfolding Rate at Clamp Force [s-¹] F05 [pN]
Actinomyces fimbrial adhesin FimA 3QDH Mainly Beta500
0.00e+00100
N/AN/A14.2N/A20Danature pulse, buffer: calcium (10 mM) to EDTA (1 mM)272200-472 Actinomyces naeslundii No CELL ADHESIONAFMconstant force DOI 2011-08-24 Devarajan, B., Krishnan, V., Narayana, S.V.L. Daniel J. Echelman, Jorge Alegre-Cebollada, Carmen L. Badilla, and Julio M. Fernández - 2016 N_U[14] 0.58
bovine carbonic anhydrase B (CAB) (it is also called bovine carbonic dehydratase II) under native conditions (with knot) 1V9E All beta proteins1700200
1.50e+02
N/AN/A13N/AN/Aa 50 mM Tris SO4^2+ buffer at pH 7.32611-261 Bos taurus NoLYASEAFMconstant velocity DOI 2004-02-10 Saito, R., Sato, T., Ikai, A., Tanaka, N. T Wang, H Arakawa, A Ikai - 2001 N_U[13]
bovine carbonic anhydrase B (CAB) (it is also called bovine carbonic dehydratase II) without knot (knotless) 1V9E All beta proteins2001
1.50e+02
N/AN/A110N/AN/Aa 50 mM Tris SO4^2+ buffer at pH 7.32611-261 Bos taurus NoLYASEAFMconstant velocity DOI 2004-02-10 Saito, R., Sato, T., Ikai, A., Tanaka, N. T Wang, H Arakawa, A Ikai - 2001 N_U[110]
Phytochrome carrying its covalently bound tetrapyrrole chromophore (DrBphP CBD) 2O9C Alpha and beta proteins (a+b)731
1.00e+07
N/AN/A104N/AN/Aff03 force field at fixed pressure (1 bar) and temperature (300 K), NPT ensemble, periodic boundary, constant velocity4-322 Deinococcus radiodurans YesTRANSFERASEall atom SMD simulationconstant velocity DOI 2007-03-06 Wagner, J.R., Brunzelle, J.S., Vierstra, R.D., Forest, K.T. Thomas Bornschlogl, David M. Anstrom, Elisabeth Mey, Joachim Dzubiella, Matthias Rief, Katrina T. Forest - 2008 N_U[104]
Phytochrome (apo-DrCBD) 2O9C Alpha and beta proteins (a+b)471
1.00e+03
N/AN/A98.1N/AN/A0.5 mg/mL in phosphate-buffered saline4-322 Deinococcus radiodurans YesTRANSFERASEAFMconstant velocity DOI 2007-03-06 Wagner, J.R., Brunzelle, J.S., Vierstra, R.D., Forest, K.T. Thomas Bornschlogl, David M. Anstrom, Elisabeth Mey, Joachim Dzubiella, Matthias Rief, Katrina T. Forest - 2008 N_U[98]
R16 repeat of Spectrin 1AJ3 All alpha proteins 70 10
 3000
N/AN/A31N/A20buffer 50 mM Tris HCl pH 8.0, 50 mM NaCl1061-106 Gallus gallusYesCYTOSKELETONAFMconstant velocity DOI 1997-07-07 Pascual, J., Pfuhl, M., Walther, D., Saraste, M., Nilges, M. P.-F Lenne, A.J Raae, S.M Altmann, M Saraste, J.K.H Horber - 2000 N/AN_I[15.5]_U[31]
Alpha 21 domain of Human Spectrin 1S35 All alpha proteins271
1.00e+03
N/AN/A411.0E-523PBS buffer1111063-1264 Homo sapiens YesSTRUCTURAL PROTEINAFMconstant velocity DOI 2004-04-20 Kusunoki, H., MacDonald, R.I., Mondragon, A. Richard Law, Philippe Carl, Sandy Harper, Paul Dalhaimer, David W. Speicher, Dennis E. Discher - 2003 N_U[41]
Alpha-actin | ACTININ 1HCI All alpha proteins38
3.00e+02
N/AN/AN/A20PBS7461-746 Homo sapiens NoTRIPLE-HELIX COILED COILAFMconstant velocity DOI 2001-06-27 Ylanne, J., Scheffzek, K., Young, P., Saraste, M. Richard Law, Sandy Harper, David W Speicher, Dennis E Discher - 2004
wild-type Beta2-microglobulin (B2m) 1LDS All beta proteins14627
2.18e+03
N/AN/A14N/A20PBS 10 mM981-99 Homo sapiens NoIMMUNE SYSTEMAFMconstant velocity DOI 1998-04-08Trinh, C.H., Smith, D.P., Kalverda, A.P., Phillips, S.E.V., Radford, S.E. B. Sorce, S. Sabella, M. Sandal, B. Samori, A. Santino, R. Cingolani, R. Rinaldi, P. P. Pompa - 2009 NAN_U[14]
Mutant of Beta2-microglobulin (B2m): Deletion of 6 N-ter amino acids 1LDS All beta proteins8017
2.18e+03
N/AN/A11.5N/A20PBS 10 mM921-93 Homo sapiensYesIMMUNE SYSTEMAFMconstant velocity DOI 1998-04-08Trinh, C.H., Smith, D.P., Kalverda, A.P., Phillips, S.E.V., Radford, S.E. B. Sorce, S. Sabella, M. Sandal, B. Samori, A. Santino, R. Cingolani, R. Rinaldi, P. P. Pompa - 2009 NAN_U[11.5]
GFP polyprotein linked by cysteine on 3-132| 1EMB: GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG 1EMB Alpha and beta proteins (a+b)35010
3.60e+03
N/AN/A41.6N/AN/APBS buffer (pH 7.4) at high protein concentrations of ~5 mg/ml (~0.2 mM) - Different pulling directions: see more in name column2292-229 Aequorea victoria YesFLUORESCENT PROTEINAFMconstant velocity DOI 1997-06-16 Brejc, K., Sixma, T. Hendrik Dietz and Matthias Rief - 2006 N/AN_I[39.3]_U[72.08]
Protein ribonuclease HI Q4C/V155C of E.coli 2YV0 Alpha and beta proteins (a/b) 18 1
 300 53
See more
13
N/A5.1410.0048520250 mM NaCl, 10 mM Tris, pH 7.0, and 1 mM EDTA1551-155 Escherichia coli Yes HYDROLASEOptical Tweezers (OT)constant force DOI 2008-03-18 Haruki, M., Motegi, T., Tadokoro, T., Koga, Y., Takano, K., Kanaya, S. Ciro Cecconi, Elizabeth A Shank, Carlos Bustamante, Susan Marqusee - 2005 N_I_I_U[41]
full titin uniprotID: Q8WZ42 All beta proteins 13010
2.50e+02138344000
N/A4.3911000.005520buffer 50 mM Tris HCl pH 8.0, 50 mM NaCl300001-30000 Homo sapiens NoIMMUNOGLOBULIN-LIKE DOMAINOptical Tweezers (OT)constant force DOI 1996-07-11 Improta, S., Politou, A.S., Pastore, A. L Tskhovrebova, J Trinick, J A Sleep, R M Simmons - 1997 N_U[1100]
Barnase 1BNI Alpha and beta proteins (a+b)26940
1.00e+09
N/A10.2383.37E-5N/AENCAD force field described in M. Levitt et al.1995, simulation at two pulling speeds: low rate where pulling speed= 0.01 angstrom/ps and high rate where pulling speed=0.02 angtrom/ps1101-110 Bacillus amyloliquefaciens NoMICROBIAL RIBONUCLEASEall atom SMD simulationconstant velocity DOI 1995-07-31 Bycroft, M. Robert B. Best, Bin Li, Annette Steward, Valerie Daggett, and Jane Clarke - 2001 N/AN_U[38]
Barnase linked to I27 | 1BNR: BARNASE 1BNR Alpha and beta proteins (a+b)70
1.00e+0230
N/A9.8383.37E-5N/APBS: 10 mM phosphate buffer, 137 mM NaCl, 2.7 mM KCl), with the addition of 0.01% sodium azide to prevent bacterial and fungal growth1101-110 Bacillus amyloliquefaciens NoMICROBIAL RIBONUCLEASEAFMconstant velocity DOI 1995-07-31 Bycroft, M. Robert B. Best, Bin Li, Annette Steward, Valerie Daggett, and Jane Clarke - 2001 N/AN_U[38]
mutant C426A/D595A of CnaB domain and TED from Pilus-presented adhesin, Spy0125 (Cpa), P212121 form (DLS) 2XID Mainly Beta35046
4.00e+02100
N/AN/A107N/AN/ApH 7.2 with 10 m M Hepes, 150 m M NaCl, and 1 m M EDTA307290-597 Streptococcus pyogenesyes CELL ADHESIONAFMconstant force DOI 2010-08-04 Pointon, J.A., Smith, W.D., Saalbach, G., Crow, A., Kehoe, M.A., Banfield, M.J. Daniel J. Echelman, Alex Q. Lee, Julio M. Fernandez - 2017 N_I[55]_U[107] 8.0E-5
1st domain of synaptotagmin I (C2A) 1DQV All beta proteins60
6.00e+02
N/AN/A38N/AN/A0.1 M imidazole PH 6.0128295-423 Rattus rattus NoENDOCYTOSIS/EXOCYTOSISAFMconstant velocity DOI 2000-01-19 Sutton, R.B., Ernst, J.A., Brunger, A.T. Mariano Carrion-Vazquez*, Andres F. Oberhauser, Thomas E. Fisher, Piotr E. Marszalek, Hongbin Li, Julio M. Fernandez - 2000 [A-G]N_U[38]
domain 4 of ddFLN 1WLH All beta proteins751
2.00e+02
N/AN/A16.91.0E-5N/APDB and room temperature100547-647 Dictyostelium discoideum NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2004-10-05 Popowicz, G.M., Mueller, R., Noegel, A.A., Schleicher, M., Huber, R., Holak, T.A. Hendrik Dietz and Matthias Rief - 2004 N_I[15]_U[16]
Domain 12 of Fibronectin 1FNF All beta proteins12518
4.00e+02
N/AN/A31N/A20PBS containing 5 mM DTT and 0.2 mM EDTA921833-1925 Homo sapiens NoHEPARIN AND INTEGRIN BINDINGAFMconstant velocity DOI 1999-03-16 Sharma, A., Askari, J., Humphries, M., Jones, E.Y., Stuart, D.I. Andres F Oberhauser, Carmelu Badilla-Fernandez, Mariano Carrion-Vazquez, Julio M Fernandez - 2002
Cadherin-23 EC1-27 and Protocadherin-15 EC1-2 (handshake complex) in 'partner-assisted pulling-PAP ' mode 4AQ8 All beta proteins241
2.00e+030
N/A1833.2N/AN/A10 mM HEPES, 50 mM KCl, 10 mM NaCl, and 50 µM CaCl 21-210 Mus musculus No CELL ADHESIONAFMconstant velocity DOI 2012-11-07 Sotomayor, M., Weihofen, W., Gaudet, R., Corey, D.P. Nisha Arora, Jagadish Prasad Hazra, and Sabyasachi Rakshit - 2021 N_I[6.5]_I[16.9]_I[24.6]_U[33.2]
calmodulin | FREE CALMODULIN 1CFC All alpha proteins0
6.00e+02
N/AN/A194N/AN/A0.1 M Tris PH 7.51481-148 Xenopus laevis NoCALCIUM-BINDING PROTEINAFMconstant velocity DOI 1995-12-07 Kuboniwa, H., Tjandra, N., Grzesiek, S., Ren, H., Klee, C.B., Bax, A. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2000 No unfolding peaks: force below 20pNN/A
construct of 6 Ankyrin repeats(NI6C) and polyprotein I27 of titin :(I27)(I27)(I27)-NI6C 1N11 Alpha and beta proteins (a+b)6020
5.00e+02
N/AN/AN/AN/AN/A253405-812 Homo sapiens NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2002-12-11 Michaely, P., Tomchick, D.R., Machius, M., Anderson, R.G.W. Whasil Lee, Xiancheng Zeng, Kristina Rotolo, Ming Yang, Christopher J. Schofield, Vann Bennett, Weitao Yang, and Piotr E. Marszalek - 2012 N_U[8.8]
6 repeats of Ankyrin (N6C) ; from second to seventh repeat 1N11 Alpha and beta proteins (a+b)3720
5.00e+02
N/AN/A11.5N/A20N/A33405-812 Homo sapiens NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2002-12-11 Michaely, P., Tomchick, D.R., Machius, M., Anderson, R.G.W. Lewyn Li, Svava Wetzel, Andreas Pluckthun, Julio M. Fernandez - 2005 N_U[11.5]
lipoyl domain of the dihydrolipoyl acetyltransferase subunit from Escherichia coli (E2lip3(-)): pulling from C-ter to N-ter 1QJO All beta proteins1773
7.00e+02
N/AN/A24.20.007620different directions of pulling: here from C-ter to N-ter791-80 Escherichia coli BL21(DE3) NoTRANSFERASEAFMconstant velocity DOI 1999-06-30 Jones, D.D., Howard, M.J., Perham, R.N. David J Brockwell, Emanuele Paci, Rebecca C Zinober, Godfrey S Beddard, Peter D Olmsted, D Alastair Smith, Richard N Perham, Sheena E Redford - 2003 N_U[24.2]
mutant C426A/D595A of CnaB domain and TED from Pilus-presented adhesin, Spy0125 (Cpa), P212121 form (DLS) 2XID Mainly Beta25446
4.00e+02
N/AN/A119N/AN/ApH 7.2 with 10 m M Hepes, 150 m M NaCl, and 1 m M EDTA307290-597 Streptococcus pyogenesyes CELL ADHESIONAFMconstant force DOI 2010-08-04 Pointon, J.A., Smith, W.D., Saalbach, G., Crow, A., Kehoe, M.A., Banfield, M.J. Daniel J. Echelman, Alex Q. Lee, Julio M. Fernandez - 2017 N_U[119]
SdrG B1 in the absence of Calicum 1R17 All beta proteins650
3.40e+030
0.071N/A360.0082N/A10 mM of EDTA (No Calcium)320276-596 Staphylococcus epidermidis No CELL ADHESIONAFMconstant velocity DOI 2003-10-28 Ponnuraj, K., Bowden, M.G., Davis, S., Gurusiddappa, S., Moore, D., Choe, D., Xu, Y., Hook, M., Narayana, S.V.L. Lukas F. Milles, Eduard M. Unterauer1 , Thomas Nicolaus1 and Hermann E. Gaub - 2018 N_U[36]
SdrG B1 in presence of Calcium 1R17 All beta proteins2000
3.40e+03317500
0.083N/A360.0082N/A10 mM of Calcium320276-596 Staphylococcus epidermidis No CELL ADHESIONAFMconstant velocity DOI 2003-10-28 Ponnuraj, K., Bowden, M.G., Davis, S., Gurusiddappa, S., Moore, D., Choe, D., Xu, Y., Hook, M., Narayana, S.V.L. Lukas F. Milles, Eduard M. Unterauer1 , Thomas Nicolaus1 and Hermann E. Gaub - 2018 N_U[36]
Cadherin-23 EC1-27 and Protocadherin-15 EC1-2 (handshake complex) in 'handle (or hook)-assisred pulling (HAP)' mode 4AQ8 All beta proteins195
2.00e+03
0.0881733.7N/AN/Ahandle (or hook)-assisred pulling (HAP) mode, refer to figure (by clicking on protein's name), buffer :10 mM HEPES, 50 mM KCl, 10 mM NaCl, and 50 uM CaCl 2 1-210 Mus musculus No CELL ADHESIONAFMconstant velocity DOI 2012-11-07 Sotomayor, M., Weihofen, W., Gaudet, R., Corey, D.P. Nisha Arora, Jagadish Prasad Hazra, and Sabyasachi Rakshit - 2021 N_I[6.4]_I[15.9]_I[24.5]_U[33.7]
Rubredoxin 1BRF Small proteins20040
4.00e+020
0.11N/A130.15N/Aroom temperature in Tris buffer at pH 7.4531-53 Pyrococcus furiosus No ELECTRON TRANSPORTAFMconstant velocity DOI 1998-09-02 Bau, R., Rees, D.C., Kurtz, D.M., Scott, R.A., Huang, H., Adams, M.W.W., Eidsness, M.K. Peng Zheng and Hongbin Li - 2011 N_U[13]
GFP pulling from residue 117 and residue 182 | 1EMB: GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG 1EMB Alpha and beta proteins (a+b)54857
3.60e+03
0.1231725.0E-5N/APBS buffer (pH 7.4) at high protein concentrations of ~5 mg/ml (~0.2 mM) - Different pulling directions: see more in name column2292-229 Aequorea victoria YesFLUORESCENT PROTEINAFMconstant velocity DOI 1997-06-16 Brejc, K., Sixma, T. Hendrik Dietz, Felix Berkemeier, Morten Bertz, and Matthias Rief - 2006 N/AN_I[39.3]_U[72.08]
GFP pulling from residue 3 and residue 132 | 1EMB: GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG 1EMB Alpha and beta proteins (a+b) 407 45
 12000
0.12526720.004N/APBS buffer (pH 7.4) at high protein concentrations of ~5 mg/ml (~0.2 mM) - Different pulling directions: see more in name column2292-229 Aequorea victoria YesFLUORESCENT PROTEINAFMconstant velocity DOI 1997-06-16 Brejc, K., Sixma, T. Hendrik Dietz, Felix Berkemeier, Morten Bertz, and Matthias Rief - 2006 N/AN_I[39.3]_U[72.08]
scaffoldin c7A | 1AOH: SINGLE COHESIN DOMAIN FROM THE SCAFFOLDING PROTEIN CIPA OF THE CLOSTRIDIUM THERMOCELLUM CELLULOSOME 1AOH All beta proteins136050
1.00e+12100
0.13121.949.36.0E-620CHARMM22 force fiels, water box 10 angstroms larger than the domain, neutralized by adding NaCl ions to the water box, minimization was done with a time-step of 1 fs1475-147 Acetivibrio thermocellus ATCC 27405NoSTRUCTURAL PROTEINall atom SMD simulationconstant velocity DOI 1998-07-08Alzari, P.M., Tavares, G. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-I][ A'-I ']N_U[49.3]
scaffoldin c7A | 1AOH: SINGLE COHESIN DOMAIN FROM THE SCAFFOLDING PROTEIN CIPA OF THE CLOSTRIDIUM THERMOCELLUM CELLULOSOME 1AOH All beta proteins71050
1.00e+11100
0.13121.949.36.0E-620CHARMM22 force fiels, water box 10 angstroms larger than the domain, neutralized by adding NaCl ions to the water box, minimization was done with a time-step of 1 fs1475-147 Acetivibrio thermocellus ATCC 27405NoSTRUCTURAL PROTEINall atom SMD simulationconstant velocity DOI 1998-07-08Alzari, P.M., Tavares, G. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-I][ A'-I ']N_U[49.3]
scaffoldin c7A | 1AOH: SINGLE COHESIN DOMAIN FROM THE SCAFFOLDING PROTEIN CIPA OF THE CLOSTRIDIUM THERMOCELLUM CELLULOSOME 1AOH All beta proteins48014
4.00e+02100
0.13121.949.36.0E-620PBS 0.2 mM EDTA 5mM DTT buffer1475-147 Acetivibrio thermocellus ATCC 27405 NoSTRUCTURAL PROTEINAFMconstant velocity DOI 1998-07-08 Alzari, P.M., Tavares, G. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-I][ A'-I ']N_U[49.3]
scaffoldin c1C | 1G1K: COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM 1G1K All beta proteins4259
4.00e+02100
0.13322.348.50.000220PBS 0.2 mM EDTA 5 mM DTT buffer1432-143 Ruminiclostridium cellulolyticum NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2000-11-22 Spinelli, S., Fierobe, H.-P., Belaich, A., Belaich, J.-P., Henrissat, B., Cambillau, C. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-I][A'-I']N_U[48.5]
scaffoldin c1C | 1G1K: COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM 1G1K All beta proteins63070
1.00e+09100
0.13322.348.50.000220CHARMM22 force fiels, water box 10 angstroms larger than the domain, neutralized by adding NaCl ions to the water box, minimization was done with a time-step of 1 fs1432-143 Ruminiclostridium cellulolyticum NoSTRUCTURAL PROTEINall atom SMD simulationconstant velocity DOI 2000-11-22 Spinelli, S., Fierobe, H.-P., Belaich, A., Belaich, J.-P., Henrissat, B., Cambillau, C. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-I][A'-I']N_U[48.5]
scaffoldin c1C | 1G1K: COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM 1G1K All beta proteins16109
1.00e+10
0.13322.348.50.000220CHARMM22 force fiels, water box 10 angstroms larger than the domain, neutralized by adding NaCl ions to the water box, minimization was done with a time-step of 1 fs1432-143 Ruminiclostridium cellulolyticumNoSTRUCTURAL PROTEINall atom SMD simulationconstant velocity DOI 2000-11-22Spinelli, S., Fierobe, H.-P., Belaich, A., Belaich, J.-P., Henrissat, B., Cambillau, C Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-I][A'-I']N_U[48.5]
GFP pulling from residue 182 and residue 212 | 1EMB: GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG 1EMB Alpha and beta proteins (a+b)35661
3.60e+03
0.1427720.0022N/APBS buffer (pH 7.4) at high protein concentrations of ~5 mg/ml (~0.2 mM) - Different pulling directions: see more in name column2292-229 Aequorea victoria YesFLUORESCENT PROTEINAFMconstant velocity DOI 1997-06-16 Brejc, K., Sixma, T. Hendrik Dietz, Felix Berkemeier, Morten Bertz, and Matthias Rief - 2006 N/AN_I[39.3]_U[72.08]
Protein G domain B1 in the presence of glycerol 1PGA Alpha and beta proteins (a+b)25050
1.00e+03
0.1531.8416.80.0059N/A10 mM Tris/HCl and 30% v/v glycerol (pH 7.5)561-56 Streptococcus sp. GX7805 No IMMUNOGLOBULIN BINDING PROTEINAFMconstant velocity DOI 1994-04-30 Gallagher, T., Alexander, P., Bryan, P., Gilliland, G.L. Daniel Aioanei, Isabella Tessari, Luigi Bubacco, Bruno Samori, Marco Brucale - 2011 N_U[16.8]
Protein G domain B1 in the absence of glycerol 1PGA Alpha and beta proteins (a+b)19050
1.00e+03
0.1650.8416.80.0372N/ATris/HCl buffer (10 mM, pH 7.5)561-56 Streptococcus sp. GX7805 NoIMMUNOGLOBULIN BINDING PROTEINAFMconstant velocity DOI 1994-04-30 Gallagher, T., Alexander, P., Bryan, P., Gilliland, G.L. Daniel Aioanei, Isabella Tessari, Luigi Bubacco, Bruno Samori, Marco Brucale - 2011 N_U[16.8]
I27 of titin (also known as I91) 1TIT All beta proteins37080
1.00e+11
0.16817.327.30.005520CHARMM22 force fiels, water box 10 angstroms larger than the domain, neutralized by adding NaCl ions to the water box, minimization was done with a time-step of 1 fs891-89 Homo sapiens NoIMMUNOGLOBULIN-LIKE DOMAINall atom SMD simulationconstant velocity DOI 1996-07-11 Improta, S., Politou, A.S., Pastore, A. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-B][A'-G]N_U[27.3]
I27 of titin (also known as I91) 1TIT All beta proteins91050
1.00e+12
0.16817.327.30.005520CHARMM22 force fiels, water box 10 angstroms larger than the domain, neutralized by adding NaCl ions to the water box, minimization was done with a time-step of 1 fs891-89 Homo sapiens NoIMMUNOGLOBULIN-LIKE DOMAINall atom SMD simulationconstant velocity DOI 1996-07-11 Improta, S., Politou, A.S., Pastore, A. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-B][A'-G]N_U[27.3]
I27 of titin (also known as I91) 1TIT All beta proteins2111
1.00e+03
0.16817.327.30.005520PBS 0.2 mM EDTA 5mM DTT buffer891-89 Homo sapiens NoIMMUNOGLOBULIN-LIKE DOMAINAFMconstant velocity DOI 1996-07-11 Improta, S., Politou, A.S., Pastore, A. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-B][A'-G]N_U[27.3]
2nd Domain of Fibronection III (FNIII) 1OWW All beta proteins22018
6.00e+02
0.170.004310.02220PBS containing 5 mM DTT and 0.2 mM EDTA91739-830 Homo sapiens NoCELL ADHESION PROTEINAFMconstant velocity DOI 1996-01-29 Leahy, D.J., Aukhil, I., Erickson, H.P. Lewyn Li 1, Hector Han-Li Huang, Carmen L Badilla, Julio M Fernandez - 2004 [A-B][A-G]N_U[31]
scaffoldin c2A | Cohesin 2 DOMAIN OF THE CELLULOSOME FROM CLOSTRIDIUM THERMOCELLUM 1ANU All beta proteins86050
1.00e+12
0.1718.448.60.02120CHARMM22 force fiels, water box 10 angstroms larger than the domain, neutralized by adding NaCl ions to the water box, minimization was done with a time-step of 1 fs1381-138 Acetivibrio thermocellusNoCOHESINall atom SMD simulationconstant velocity DOI 1997-07-23Shimon, L.J.W., Yaron, S., Shoham, Y., Lamed, R., Morag, E., Bayer, E.A., Frolow, F. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-I][ A'-I ']N_U[48.6]
1st domain of Fibronectin III (FNIII) 1FNH All beta proteins20018
6.00e+02
0.1720.5280.02220PBS containing 5 mM DTT and 0.2 mM EDTA97625-722 Homo sapiens NoCELL ADHESION PROTEINAFMconstant velocity DOI 1996-01-29 Leahy, D.J., Aukhil, I., Erickson, H.P. Andres F Oberhauser, Carmelu Badilla-Fernandez, Mariano Carrion-Vazquez, Julio M Fernandez - 2002 [A-B][A-G]N_I[20]_U[28]
scaffoldin c2A | Cohesin 2 DOMAIN OF THE CELLULOSOME FROM CLOSTRIDIUM THERMOCELLUM 1ANU All beta proteins47080
1.00e+11
0.1718.448.60.02120CHARMM22 force fiels, water box 10 angstroms larger than the domain, neutralized by adding NaCl ions to the water box, minimization was done with a time-step of 1 fs1381-138 Acetivibrio thermocellusNoCOHESINall atom SMD simulationconstant velocity DOI 1997-07-23Shimon, L.J.W., Yaron, S., Shoham, Y., Lamed, R., Morag, E., Bayer, E.A., Frolow, F. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-I][ A'-I ']N_U[48.6]
scaffoldin c2A | Cohesin 2 DOMAIN OF THE CELLULOSOME FROM CLOSTRIDIUM THERMOCELLUM 1ANU All beta proteins2148
4.00e+02100
0.1718.448.60.02120PBS 0.2 mM EDTA 5 mM DTT buffer1381-138 Acetivibrio thermocellus NoCOHESINAFMconstant velocity DOI 1997-07-23 Shimon, L.J.W., Yaron, S., Shoham, Y., Lamed, R., Morag, E., Bayer, E.A., Frolow, F. Alejandro Valbuena, Javier Oroz, Ruben Hervas, and Mariano Carrion-Vazquez - 2009 [A-I][ A'-I ']N_U[48.6]
protein L | 1HZ6: CRYSTAL STRUCTURES OF THE B1 DOMAIN OF PROTEIN L FROM PEPTOSTREPTOCOCCUS MAGNUS WITH A TYROSINE TO TRYPTOPHAN SUBSTITUTION 1HZ6 Alpha and beta proteins (a+b)500200
4.00e+09
0.22N/A18.80.0520N/A622-64 Finegoldia magna ATCC 29328 YesPROTEIN BINDINGall atom SMD simulationconstant velocity DOI 2001-04-04 O'Neill, J.W., Kim, D.E., Baker, D., Zhang, K.Y.J. David J. Brockwell, Godfrey S. Beddard, Emanuele Paci, Peter D. Olmsted, D. Alastair Smith, and Sheena E Redford - 2005 N_U[18.8]
protein L | 1HZ6: CRYSTAL STRUCTURES OF THE B1 DOMAIN OF PROTEIN L FROM PEPTOSTREPTOCOCCUS MAGNUS WITH A TYROSINE TO TRYPTOPHAN SUBSTITUTION 1HZ6 Alpha and beta proteins (a+b) 91 3
 40
0.22N/A18.80.0520PBS622-64 Finegoldia magna ATCC 29328 YesPROTEIN BINDINGAFMconstant velocity DOI 2001-04-04 O'Neill, J.W., Kim, D.E., Baker, D., Zhang, K.Y.J. David J. Brockwell, Godfrey S. Beddard, Emanuele Paci, Peter D. Olmsted, D. Alastair Smith, and Sheena E Redford - 2005 N_U[18.8]
Native Ubiquitin at PH=2 1UBQ All alpha proteins18034
1.00e+03
0.225N/A24.50.02N/Aforces were measured at different PH, here PH=2. buffer PBS: 126 mM NaCl, 7.2 mM Na2HPO4, 3 mM NaH2PO41-76 Gallus gallus NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2004-10-19 Kusunoki, H., Minasov, G., MacDonald, R.I., Mondragon, A. Chia-Lin Chyan, Fan-Chi Lin, Haibo Peng, Jian-Min Yuan, Chung-Hung Chang, Sheng-Hsien Lin, and Guoliang Yang - 2004 N_U[24]
Native Ubiquitin at PH from 6 to 10 1UBQ All alpha proteins 230 34
 1000
0.225N/A24.55.0E-5N/Aforces were measured at different PH, here PH from 6 to 10. buffer PBS: 126 mM NaCl, 7.2 mM Na2HPO4, 3 mM NaH2PO41-76 Gallus gallus NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2004-10-19 Kusunoki, H., Minasov, G., MacDonald, R.I., Mondragon, A. Chia-Lin Chyan, Fan-Chi Lin, Haibo Peng, Jian-Min Yuan, Chung-Hung Chang, Sheng-Hsien Lin, and Guoliang Yang - 2004 N_U[24]
N-C linked ubiquitin 1UBQ Alpha and beta proteins (a+b)200035
1.00e+11
0.25N/A240.0004N/A CHARMM22 force field, NPT ensemble at contant velocity, periodic boundary condition761-76 Homo sapiens NoCHROMOSOMAL PROTEINall atom SMD simulationconstant velocity DOI 1987-04-16 Vijay-Kumar, S., Bugg, C.E., Cook, W.J. Mariano Carrion-Vazquez 1 , Hongbin Li, Hui Lu, Piotr E Marszalek, Andres F Oberhauser, Julio M Fernandez - 2003 N_U[24]
I27 of titin (also known as I91) 1TIT All beta proteins 225 1
 1000
0.25N/A28.70.0055N/A25 mM imidazole-HCl, pH 7.4, 0.2M KCI,4mM891-89 Homo sapiens NoIMMUNOGLOBULIN-LIKE DOMAINAFMconstant velocity DOI 1996-07-11 Improta, S., Politou, A.S., Pastore, A. Kaori Watanabe, Claudia Muhle-Goll, Miklos S Z Kellermayer, Siegfried Labeit, Henk Granzier - 2002 [A-B][A'-G]N_U[28.7]
N-C linked ubiquitin 1UBQ Alpha and beta proteins (a+b)20335
4.00e+02
0.25N/A240.0004N/Aexamination of the mechanical stability of N-C linked. buffer: 50 mM Tris, pH 7.6761-76 Homo sapiens NoCHROMOSOMAL PROTEINAFMconstant velocity DOI 1987-04-16 Vijay-Kumar, S., Bugg, C.E., Cook, W.J. Mariano Carrion-Vazquez, Hongbin Li, Hui Lu, Piotr E Marszalek, Andres F Oberhauser, Julio M Fernandez - 2003 N_U[24]
lipoyl domain of the dihydrolipoyl acetyltransferase subunit from Escherichia coli (E2lip3(+)): pulling from C-ter to Residue 41 (Lys) 1QJO All beta proteins1873
7.00e+02
0.29N/A24.20.007620different directions of pulling: here from C-ter to Lys 41791-80 Escherichia coli BL21(DE3) NoTRANSFERASEAFMconstant velocity DOI 1999-06-30 Jones, D.D., Howard, M.J., Perham, R.N. David J Brockwell, Emanuele Paci, Rebecca C Zinober, Godfrey S Beddard, Peter D Olmsted, D Alastair Smith, Richard N Perham, Sheena E Redford - 2003 N_I[10.9]_U[24.2]
GFP(132,212) :from copolymers with mixed linkage geometries: (3, 212) and (132, 212)| 1EMB: GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG 1EMB Alpha and beta proteins (a+b)11722
3.60e+03
0.3225720.014N/APBS buffer (pH 7.4) at high protein concentrations of ~5 mg/ml (~0.2 mM) - Different pulling directions: see more in name column2292-229 Aequorea victoriaYesFLUORESCENT PROTEINAFMconstant velocity DOI 1997-06-16Brejc, K., Sixma, T. Hendrik Dietz and Matthias Rief - 2006 N/AN_I[39.3]_U[72.08]
GFP polyprotein linked by cysteine on 132-212| 1EMB: GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG 1EMB Alpha and beta proteins (a+b)11722
3.60e+03
0.3225720.014N/APBS buffer (pH 7.4) at high protein concentrations of ~5 mg/ml (~0.2 mM) - Different pulling directions: see more in name column2292-229 Aequorea victoria YesFLUORESCENT PROTEINAFMconstant velocity DOI 1997-06-16 Brejc, K., Sixma, T. Hendrik Dietz, Felix Berkemeier, Morten Bertz, and Matthias Rief - 2006 N/AN_I[39.3]_U[72.08]
GFP pulling from residue 132 and residue 212 | 1EMB: GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG 1EMB Alpha and beta proteins (a+b)12722
3.60e+03
0.3226720.005N/APBS buffer (pH 7.4) at high protein concentrations of ~5 mg/ml (~0.2 mM) - Different pulling directions: see more in name column2292-229 Aequorea victoriaYesFLUORESCENT PROTEINAFMconstant velocity DOI 1997-06-16Brejc, K., Sixma, T. Hendrik Dietz, Felix Berkemeier, Morten Bertz, and Matthias Rief - 2006 N/AN_I[39.3]_U[72.08]
Domain 13 of Fibronectin III (FNIII) 1FNF All beta proteins8918
6.00e+02
0.3419.5280.02220PBS containing 5 mM DTT and 0.2 mM EDTA891926-2015 Homo sapiens NoCELL ADHESION PROTEINAFMconstant velocity DOI 1996-01-29 Leahy, D.J., Aukhil, I., Erickson, H.P. Andres F Oberhauser, Carmelu Badilla-Fernandez, Mariano Carrion-Vazquez, Julio M Fernandez - 2002 [A-B][A-G]N_U[31]
I1 DOMAIN OF TITIN 1G1C All beta proteins12718
6.00e+02
0.35N/A300.005N/APBS buffer1001-100 Homo sapiens NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2001-10-12 Mayans, O., Wuerges, J., Gautel, M., Wilmanns, M. Hongbin Li, Julio M Fernandez - 2003 [A-B A'-G]N_U[30]
I65 to I70 Igs of titin 3B43 All beta proteins 200
200
0.35N/A31.91.0E-52025 mM imidazole-HCl, PH 7.4, 0.2 M KCl, 4mM MgCl2, 1mM EGTA, 0.01% NaN3, 5mM DTT5721-573 Oryctolagus cuniculus No STRUCTURAL PROTEINAFMconstant velocity DOI 2008-01-22 von Castelmur, E., Marino, M., Labeit, D., Labeit, S., Mayans, O. Kaori Watanabe, Claudia Muhle-Goll, Miklós S Z Kellermayer, Siegfried Labeit, Henk Granzier - 2002 [A-B][A'-G]N_U[31.9]
V17P mutant of I1 domain of TITIN 1G1C All beta proteins11742
6.00e+02
0.35N/A300.008N/APBS buffer1001-100 Homo sapiens NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2001-10-12 Mayans, O., Wuerges, J., Gautel, M., Wilmanns, M. Hongbin Li, Julio M Fernandez - 2003 [A-B A'-G]N_U[30]
Q15P mutant of I1 domain of TITIN 1G1C All beta proteins8228
6.00e+02
0.35N/A300.05N/APBS buffer1001-100 Homo sapiens NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2001-10-12 Mayans, O., Wuerges, J., Gautel, M., Wilmanns, M. Hongbin Li, Julio M Fernandez - 2003 [A-B A'-G]N_U[30]
R28P mutant of I1 domain of TITIN 1G1C All beta proteins8228
6.00e+02
0.35N/A300.05N/APBS buffer1001-100 Homo sapiens NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2001-10-12 Mayans, O., Wuerges, J., Gautel, M., Wilmanns, M. Hongbin Li, Julio M Fernandez - 2003 [A-B A'-G]N_U[30]
E4P mutant domain I1 of TITIN 1G1C All beta proteins8023
6.00e+02
0.35N/A300.07N/APBS buffer1001-100 Homo sapiens NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2001-10-12 Mayans, O., Wuerges, J., Gautel, M., Wilmanns, M. Hongbin Li, Julio M Fernandez - 2003 [A-B A'-G]N_U[30]
K7P mutant of domain I1 of TITIN 1G1C All beta proteins6930
6.00e+02
0.35N/A300.1N/APBS buffer1001-100 Homo sapiens NoSTRUCTURAL PROTEINAFMconstant velocity DOI 2001-10-12 Mayans, O., Wuerges, J., Gautel, M., Wilmanns, M. Hongbin Li, Julio M Fernandez - 2003 [A-B A'-G]N_U[30]
One step unfolding of Domain 13 of Fibronectin III (FNIII) 1FNF All beta proteins10010
3.00e+02
0.38N/A310.0220buffer 50 mM Tris HCl pH 8.0, 50 mM NaCl941559-1653 Homo sapiens NoCELL ADHESION PROTEINAFMconstant velocity DOI 1996-01-29 Leahy, D.J., Aukhil, I., Erickson, H.P. Lewyn Li 1, Hector Han-Li Huang, Carmen L Badilla, Julio M Fernandez - 2004 [A-B][A-G]N_U[31]
Two steps unfolding of Domain 13 of Fibronectin III (FNIII) 1FNF All beta proteins75
4.00e+02
0.38N/A310.0220PBS containing 5 mM DTT and 0.2 mM EDTA941559-1653 Homo sapiens NoCELL ADHESION PROTEINAFMconstant velocity DOI 1996-01-29 Leahy, D.J., Aukhil, I., Erickson, H.P. Lewyn Li, Hector Han-Li Huang, Carmen L. Badilla and Julio M. Fernandez - 2002 [A-B][A-G]N_I[12]_U[31]
WildType bacteriodepsin | STRUCTURE OF BACTERIORHODOPSIN AT 3.0 ANGSTROM DETERMINED BY ELECTRON CRYSTALLOGRAPHY 1AT9 All alpha proteins15050
4.00e+011000
0.4N/A40N/A20buffer solution (300 mM KCl, 10 mM tris-HCl, pH 7.8)2311-231 Halobacterium salinarum NoPHOTORECEPTORAFMconstant velocity DOI 1998-09-16 Kimura, Y., Vassylyev, D.G., Miyazawa, A., Kidera, A., Matsushima, M., Mitsuoka, K., Murata, K., Hirai, T., Fujiyoshi, Y. F Oesterhelt, D Oesterhelt, M Pfeiffer, A Engel, H E Gaub, D J Muller - 2000 [G-F & E-D][B][C]N_I[10]_I|30]_I|50]_U[70]
bacteriorhodopsin muted by cleavage of E-F loop | 1AT9: STRUCTURE OF BACTERIORHODOPSIN AT 3.0 ANGSTROM DETERMINED BY ELECTRON CRYSTALLOGRAPHY 1AT9 All alpha proteins10050
4.00e+011000
0.4N/A40N/A20buffer solution (300 mM KCl, 10 mM tris-HCl, pH 7.8)2311-231 Halobacterium salinarum NoPHOTORECEPTORAFMconstant velocity DOI 1998-09-16 Kimura, Y., Vassylyev, D.G., Miyazawa, A., Kidera, A., Matsushima, M., Mitsuoka, K., Murata, K., Hirai, T., Fujiyoshi, Y. F Oesterhelt, D Oesterhelt, M Pfeiffer, A Engel, H E Gaub, D J Muller - 2000 [D-E][B-C][A]N_I[18]_I[26]_U[40]
G241C mutant of bacteriodepsin | 1AT9: STRUCTURE OF BACTERIORHODOPSIN AT 3.0 ANGSTROM DETERMINED BY ELECTRON CRYSTALLOGRAPHY 1AT9 All alpha proteins15050
4.00e+011000
0.4N/A40N/A20buffer solution (300 mM KCl, 10 mM tris-HCl, pH 7.8)2311-231 Halobacterium salinarum NoPHOTORECEPTORAFMconstant velocity DOI 1998-09-16 Kimura, Y., Vassylyev, D.G., Miyazawa, A., Kidera, A., Matsushima, M., Mitsuoka, K., Murata, K., Hirai, T., Fujiyoshi, Y. F Oesterhelt, D Oesterhelt, M Pfeiffer, A Engel, H E Gaub, D J Muller - 2000 [F-G][D-E][B-C][A]N_I[10]_I[25]_I[45]_U[65]
GFP(3-212) :from copolymers with mixed linkage geometries: (3, 212) and (132, 212)| 1EMB: GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG 1EMB Alpha and beta proteins (a+b)11522
3.60e+03
0.4528720.0008N/APBS buffer (pH 7.4) at high protein concentrations of ~5 mg/ml (~0.2 mM) - Different pulling directions: see more in name column2292-229 Aequorea victoriaYesFLUORESCENT PROTEINAFMconstant velocity DOI 1997-06-16Brejc, K., Sixma, T. Hendrik Dietz and Matthias Rief - 2006 N/AN_I[39.3]_U[72.08]
GFP polyprotein 2-212 1EMB Alpha and beta proteins (a+b)11722
1.20e+04
0.4528720.014N/AMeasured for GFP polyprotein linked by Cysteine on 3-212 and When pulling from residue 3(N-ter) & 212(C-ter)2292-229 Aequorea victoriaYesFLUORESCENT PROTEINAFMconstant velocity DOI 1997-06-16Brejc, K., Sixma, T. Hendrik Dietz, Felix Berkemeier, Morten Bertz, and Matthias Rief - 2006 N/AN_I[39.3]_U[72.08]
The 3rd spectrin repeat of alpha-actinin-4 1WLX All alpha proteins 75 1
See more
29
8
12
26
13
6
17
20
21
12
28
50
64
 100000 1154.72
See more
2612.11
3960.57
8619.52
33273.7
52439.4
202430
694317
2.68E+06
4.22E+06
5.83E+06
9.19E+06
5.54E+07
7.66E+07
0.5318.43411N/APBS: 10 mM Na2HPO4 , 1.76 mM KH2 PO 4 , 137 mM NaCl, 2.7 mM KCl at room temperature1291-129 Homo sapiens NoPROTEIN BINDINGHigh Speed AFMconstant velocity DOI 2005-08-09 Kowalski, K., Merkel, A.L., Booker, G.W. Hirohide Takahashi, Felix Rico, Christophe Chipot, and Simon Scheuring - 2018 N_I[10]_U[34]
GFP linked to eight Ig modules (Ig27 - Ig34) | 1B9C: Green Fluorescent Protein Mutant F99S, M153T and V163A 1B9C Alpha and beta proteins (a+b)10440
3.00e+02
0.552276.6N/A2020 ul of protein solution (PBS buffer, pH 7.4; protein concentration, ~0.5 g/liter)2274-230 Aequorea victoria YesLUMINESCENT PROTEINAFMconstant velocity DOI 2000-11-17 Battistutta, R., Negro, A., Zanotti, G. Hendrik Dietz and Matthias Rief - 2004 [last alpha helix][last beta strand]N_I[3.2]_I[0.28]_I[6.5]_I[0.55]_U[69.3]
GFP linked to DdFLN1-5 [actin crosslinker dictyostelium discoideum filamin] | 1B9C: Green Fluorescent Protein Mutant F99S, M153T and V163A 1B9C Alpha and beta proteins (a+b)10440
3.00e+02
0.552276.6N/A2020 ul of protein solution (PBS buffer, pH 7.4; protein concentration, ~0.5 g/liter)2274-230 Aequorea victoria YesLUMINESCENT PROTEINAFMconstant velocity DOI 2000-11-17 Battistutta, R., Negro, A., Zanotti, G. Hendrik Dietz and Matthias Rief - 2004 [last alpha helix][last beta strand]N_I[3.2]_I[0.28]_I[6.5]_I[0.55]_U[69.3]
Lys48-C linked poly-ubiquitin 1UBQ Alpha and beta proteins (a+b)12001
1.00e+11
0.63N/A7.80.0004N/ACHARMM22 force field,NPT ensemble at contant velocity, periodic boundary condition761-76 Homo sapiens NoCHROMOSOMAL PROTEINall atom SMD simulationconstant velocity DOI 1987-04-16 Vijay-Kumar, S., Bugg, C.E., Cook, W.J. Mariano Carrion-Vazquez 1 , Hongbin Li, Hui Lu, Piotr E Marszalek, Andres F Oberhauser, Julio M Fernandez - 2003 [strand3 - strand4]N_U[7]
Lys48-C linked poly-ubiquitin 1UBQ Alpha and beta proteins (a+b)8520
3.00e+02
0.63N/A7.80.0004N/Aexamination of the mechanical stability of Lys48-C linked ubiquitin. buffer: 50 mM Tris, pH 7.6761-76 Homo sapiens NoCHROMOSOMAL PROTEINAFMconstant velocity DOI 1987-04-16 Vijay-Kumar, S., Bugg, C.E., Cook, W.J. Mariano Carrion-Vazquez, Hongbin Li, Hui Lu, Piotr E Marszalek, Andres F Oberhauser, Julio M Fernandez - 2003 [strand3 - strand4]N_U[7]
T4 Lysozyme | 1B6I: T4 LYSOZYME MUTANT WITH CYS 54 REPLACED BY THR, CYS 97 REPLACED BY ALA, THR 21 REPLACED BY CYS AND LYS 124 REPLACED BY CYS (C54T,C97A,T21C,K124C) 1B6I Alpha and beta proteins (a+b)6416
1.00e+03
0.817.6360.000120PBS1621-162 Escherichia virus T4 NoHYDROLASEScanning Force Microscope (SFM)constant velocity DOI 2000-01-12 Vetter, I.R., Baase, W.A., Snow, S., Matthews, B.W. Guoliang Yang, Ciro Cecconi, Walter A. Baase, and Carlos Bustamante - 2000
I27 of titin (also known as I91) 1TIT All beta proteins 350 1
 1000000 138344000
0.89362.0E-10N/Abuffer 50 mM Tris HCl pH 8.0, 50 mM NaCl891-89 Homo sapiens NoIMMUNOGLOBULIN-LIKE DOMAINHigh Speed AFMconstant velocity DOI 1996-07-11 Improta, S., Politou, A.S., Pastore, A. Felix Rico, Laura Gonzalez, Ignacio Casuso, Manel Puig-Vidal, and Simon Scheuring - 2013 [A-B][A'-G]
I27 of titin (also known as I91) 1TIT All beta proteins 300 1
 100000 44594100
0.89362.0E-10N/ANPT ensemble, periodic boundary conditions, constant velocity pulling891-89 Homo sapiens NoIMMUNOGLOBULIN-LIKE DOMAINall atom SMD simulationconstant velocity DOI 1996-07-11 Improta, S., Politou, A.S., Pastore, A. Felix Rico, Laura Gonzalez, Ignacio Casuso, Manel Puig-Vidal, and Simon Scheuring - 2013 [A-B][A'-G]
Vascular Adhesion Molecule 1 (VCAM1) in presence of reducing agent 1VSC All alpha proteins401
1.00e+03
1.365N/A0.000123at T = 23 C with reduction 1 mM TCEP1961-196 Mus musculus NoSTRUCTURAL GENOMICS, UNKNOWN FUNCTIONAFMconstant velocity DOI 2005-05-03 Abe, T., Hirota, H., Tomizawa, T., Koshiba, S., Kigawa, T., Yokoyama, S., RIKEN Structural Genomics/Proteomics Initiative (RSGI) Nishant Bhasin, Philippe Carl, Sandy Harper, Gang Feng, Hui Lu, David W. Speicher, Dennis E. Discher - 2004
Native Vacsular Cellular Adhesion Molecule 1 (VCAM1) 1VSC All alpha proteins401
1.00e+03
1.6N/A1.0E-723at T = 23 C and without any reducing agent1961-196 Mus musculus NoSTRUCTURAL GENOMICS, UNKNOWN FUNCTIONAFMconstant velocity DOI 2005-05-03 Abe, T., Hirota, H., Tomizawa, T., Koshiba, S., Kigawa, T., Yokoyama, S., RIKEN Structural Genomics/Proteomics Initiative (RSGI) Nishant Bhasin, Philippe Carl, Sandy Harper, Gang Feng, Hui Lu, David W. Speicher, Dennis E. Discher - 2004
Spectrin 1AJ3 All alpha proteins305
3.00e+02
1.7N/A31.30.000320PBS1061-106 Gallus gallusNoCYTOSKELETONAFMconstant velocity DOI Pascual, J., Pfuhl, M., Walther, D., Saraste, M., Nilges, M M Rief, J Pascual, M Saraste, H E Gaub - 1999 N/AN_U[31.3]